Is it possibly to run without scATAC data and only scRNA-seq?

[pcm32]

Hi there,

From what I read in the paper and in the tutorial, it seems that scATAC data is compulsory to run HuMMuS, but I thought I'd ask anyways. Thanks!

[r-trimbour]

Hello !

Thank you for your interest in HuMMuS and your question. : )

In its current implementation, HuMMuS connects TFs, DNA regions, and genes. You thus need a network of DNA regions, typically extracted from scATAC data, yes.

HuMMuS allows however to use unpaired data, or pre-computed networks. So if a scATAC dataset has been generated for the same organism/condition, you could probably use it even if it's not the same replicate or sample.