32bit images

[py-E-SIS]

Hi ,
Congratulations for MetroloJ_QC . It is great.
Nevertheless we encounter a minor but quite problematic for us issue.

The .tiff exports from our microscopes are 32-bit and the MetroloJ_QC accepts either 8 or 16 bit images.
Currently this means we need to reduce our images -> a)adding a few unnecesary steps to the workflow
b)artifically reducing the dynamic range of the image

WOuld it be possible for you to allow import of 32-bit images?

Many thanks in advance
Evangelos

[juliomateoslangerak]

Hi,
I will not be able to help you here but @julien-cau will. Just want to understand, independently of weather this is possible or not, the context of those acquisitions, I had a few questions
Are these camera based acquisitions? If yes, which cameras provide 32bit images? Which software exports raw data in 32 bits? Some kind of reconstructed data?
I guess those images would have to be converted anyway as many of ImageJ's algorithms do not work in 32bit images (no idea if this is the case for MetroloJ) but, what kind of conversion would you suggest? scaling (and loose the dynamic range) or clipping (and risk saturation) or something else?